Separation of Nanoparticles in Aqueous Multiphase Systems through Centrifugation

Separation of nanoparticles in aqueous multiphase systems through centrifugation.

This paper demonstrates the use of aqueous multiphase systems (MuPSs) as media for rate-zonal centrifugation to separate nanoparticles of different shapes and sizes. The properties of MuPSs do not change with time or during centrifugation; this stability facilitates sample collection after separation. A three-phase system demonstrates the separation of the reaction products (nanorods, nanospher...

All-aqueous multiphase microfluidics.

Immiscible aqueous phases, formed by dissolving incompatible solutes in water, have been used in green chemical synthesis, molecular extraction and mimicking of cellular cytoplasm. Recently, a microfluidic approach has been introduced to generate all-aqueous emulsions and jets based on these immiscible aqueous phases; due to their biocompatibility, these all-aqueous structures have shown great ...

Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems

Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...

Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans

Density-gradient-free microfluidic centrifugation for analytical and preparative separation of nanoparticles.

Sedimentation and centrifugation techniques are widely applied for the separation of biomolecules and colloids but require the presence of controlled density gradients for stable operation. Here we present an approach for separating nanoparticles in free solution without gradients. We use microfluidics to generate a convective flow perpendicular to the sedimentation direction. We show that the ...